HBsAg ARAŞTIRILMASINDA ELISA VE RPHA YÖNTEMLERİNİN DUYARLILIKLARI
نویسندگان
چکیده
منابع مشابه
HBsAg ELISA Kit
HBsAg is one of the earliest markers that appear in the blood following infection with Hepatitis B virus (HBV). This infection of the liver is transmitted by homosexual or heterosexual activity, blood borne exposure, mother infant, close personal contact and by intake of contaminated water and food products. In the HBV infected people, the virus persists for the rest of their lives and can be p...
متن کاملNegative Interference in Serum HBsAg ELISA from Rheumatoid Factors
BACKGROUND RF(Rheumatoid factor) is usually thought to cause positive interference in immunoassay. Recently, our study showed that high-concentration RFs caused negative interference as well as positive interference in serum HBsAg(Hepatitis B surface antigen) ELISA(Enzyme-linked immunosorbent assay), but it is unclear that RF causing negative interference is an anomaly produced by a certain ELI...
متن کاملEvaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of HbsAg.
Summi~ ry An ELlSA (enzyme-linked immunosorbent assay) method for detecting HB,Ag was compared with the RPHA (reverse passive haemagglutination) method. A total of 90 specimens were tested and i t was found that the ELlSA method was equal in sensitivity to the RPHA method; 45 out of 90 samples were positive by both ELlSA and RPHA and 42 out of 90 negative. In addition, 3 samples weakly positive...
متن کاملFalse-positive ELISA reactivity to hepatitis B virus surface antigen (HBsAg)
It is claimed that the possibility of non-specific reactions in ELISA tests for HBsAg has been substantially eliminated (1), though like any other highly sensitive immune test system, ELISA does still have the potential of yielding false-positive reactions. Such reactivity in the hepatitis B virus (HBV) hyperendemic focus of the Gizan Area of Saudi Arabia was encountered using a two-titer test ...
متن کاملDetection of Antibodies Against DNA Polymerase of Hepatitis B Virus in HBsAg-Positive Sera Using ELISA
OBJECTIVES DNA polymerase (pol) of Hepatitis B virus (HBV) includes 3 different domains such as terminal protein (TP), reverse transcriptase (RT) and RNase H. Humoral immune responses to each of these proteins have not been well documented previously, although antibody to pol was detected in serum of patients with chronic hepatitis B. We have constructed TP (amino acids 1-182), RT (amino acids ...
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ژورنال
عنوان ژورنال: Ankara Üniversitesi Tıp Fakültesi Mecmuası
سال: 1994
ISSN: 0365-8104
DOI: 10.1501/tipfak_0000000244